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KMID : 0545119940040020146
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Journal of Microbiology and Biotechnology
1994 Volume.4 No. 2 p.146 ~ p.150
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Optimization of Fed-Batch Fermentation for Production of Poly-β-Hydroxybutyrate in Alcaligenes eutrophus
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Lee, In Young
Choi, Eun Soo/Kim, Guk Jin/Nam, Soo Wan/Shin, Yong Cheol/Chang, Ho Nam/Park, Young Hoon
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Abstract
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Production of poly-β hydroxybutyrate (PHB) in fed-batch fermentation was studied. Utilixzation of carbon for PHB biosynthesis was investigated by using feeding solutions with different ratios of carbon nitrogen (C/N). It was observed that at a high C/N ratio carbon source was more favored at low C/N value. A high cell concentration (184 g/l) was achieved when ammonium hydroxide solution was fed to control the pH, which was also utilized as the sole nitrogen source. For the mass production of PHB, two-stage fed-batch operations were carried out where PHB accumulation was observed to be stimulated by switching the ammonium feeding mode to thenitrogen limiting condition. A large amount of PHB (108 g/l) was obtained with cellular content of 80% within 50 hrs of operation.
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